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BPS Bioscience
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Selleck Chemicals
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Tocris
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Chembridge
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Incyte corporation
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Lee Laboratories
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Shimadzu Corporation
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BASF
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ICI Pharmaceuticals
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ASINEX Inc
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Medicilon Inc
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BUCHI AG
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Image Search Results
Journal: EMBO Molecular Medicine
Article Title: WLS inhibits melanoma cell proliferation through the β-catenin signalling pathway and induces spontaneous metastasis
doi: 10.1002/emmm.201201486
Figure Lengend Snippet: A. Growth curves of A375 and MEL624 cells expressing GFP or WLS-GFP that were treated with 0.5 µM XAV939, a WNT/β-catenin inhibitor, (blue) or DMSO (red and black) in two-dimensional culture, p < 0.001. B,C. Soft agar assay of A375 cells stably expressing GFP or WLS-GFP. Cells were grown in the presence of either 10% control, WNT3A- or WNT5A-conditioned-media. ( B ) Representative photos of soft agar colonies stained with crystal violet after 3 weeks in culture. ( C ) Quantification of the average number of colonies formed, * p < 0.0001. D. Quantification of AXIN2 mRNA in A375 cells expressing either GFP or WLS-GFP. AXIN2 expression was normalized to GAPDH , two-way ANOVA, p < 0.0001. E. Quantification of AXIN2 mRNA in A375 cell lines treated with the WNT/β-catenin inhibitor XAV939 (0.5 µM) or DMSO control for 72 h. AXIN2 expression was normalized to GAPDH , two-way ANOVA, p < 0.0001.
Article Snippet:
Techniques: Expressing, Soft Agar Assay, Stable Transfection, Control, Staining